Cas-CLOVER. An innovative and robust gene-editing tool

Cas-CLOVER a novel gene-editing technology. it considered being more efficient and specific in contrast to CRISPR Cas 9 technology.

The potential of gene-editing technology is immense. It is necessary for creating organisms with desired characters, helps in treating genetic disorders. CRISPR stands for clustered regularly interspaced short palindromic repeats. It has been a great innovation in the science of genetics. It is the most popularly used gene-editing tool in order to create unique heredity structures of plants, animals, and microorganisms. CRISPR is considered to be the most efficient and precise tool for creating genetic modifications. But it also creates some of the off-target mutations because it uses a single RNA guide.

A new unique gene-editing tool called Cas-CLOVER was introduced in a webinar organized by Genetic Engineering and Biotechnology News recently. Technically it is fully dimeric Clo51-dCas9 genome editing technology. Cas-Clover is created by Hera Biolabs, which is practically like CRISPR-Cas9. It basically utilizes a different nuclease protein called Clo51 endonuclease. Cas-CLOVER is a combination of proteins that involves a nuclease-inactivated Cas9 protein melded to the Clo51 endonuclease. Cas-Clover requires two RNA guides with their activity-dependent on specific spacer lengths. In contrast with CRISPR, it is considered to be the most unique efficient tool, with high fidelity and robust editing efficiency. In addition, it creates no off-target mutation as demonstrated by Next Generation Sequencing (NGS). 

Concluding all of the above, the Cas-CLOVER genome editing tool is considered to be novel as well as an advanced tool. It exhibits high specificity and efficiency in both proliferating and resting t cells. It overcomes the various limitations of CRISPR and TALENS. The capability to provide higher fidelity genome editing proved to be safer in clinical applications. This new innovative technology will open new doors of research in genetic science. The future prospects of this technology could be much more.

Ahsan Ali

A young motivated person, interested in research and bioenterpreneurship in Pakistan.

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