The New Antibiotic Can Kill Even Drug-Resistant Bacteria the Antibiotic-resistant pathogens could be defeated with the assistance of a synthetic antibiotic
A brand-new antibiotic that was developed at The Rockefeller University using computational models of bacterial gene products appears to kill even bacteria that are resistant to other antibiotics. According to a study published in the journal Science, the drug, known as cilagicin, is effective in mice and employs a novel mechanism to combat MRSA, C. diff, and numerous other dangerous infections. The findings imply that computer models may be used to develop a new class of antibiotics. “This isn’t just a cool new molecule, it’s a validation of a novel approach to drug discovery,” says Rockefeller’s Sean F. Brady. “This study is an example of computational biology, genetic sequencing, and synthetic chemistry coming together to unlock the secrets of bacterial evolution.”
Bacteria have spent billions of years inventing novel methods to kill one another, so it’s not surprising that many of our most potent antibiotics originated from bacteria. With the exception of penicillin and a few other prominent antibiotics originating from fungus, the majority of antibiotics were first used as weapons by bacteria to combat other bacteria. “Eons of evolution have given bacteria unique ways of engaging in warfare and killing other bacteria without their foes developing resistance,” says Brady, the Evnin Professor and head of the Laboratory of Genetically Encoded Small Molecules. Antibiotic drug discovery once largely consisted of scientists growing streptomyces or bacillus in the lab and bottling their secrets to treat human diseases.
But with the rise of antibiotic-resistant bacteria, there is an urgent need for new active compounds and we may be running out of bacteria that are easy to exploit. Untold numbers of antibiotics, however, are likely hidden within the genomes of stubborn bacteria that are tricky or impossible to study in the lab. “Many antibiotics come from bacteria, but most bacteria can’t be grown in the lab,” Brady says. “It follows that we’re probably missing out on most antibiotics.” Finding antibacterial genes in soil and cultivating them inside more lab-friendly bacteria is an alternate strategy that has been championed by the Brady lab for the last fifteen years. But even this approach has certain drawbacks. The majority of antibiotics come from genetic sequences that are locked within bacterial gene clusters named “biosynthetic gene clusters,” which work together to collectively code for a number of proteins. But with present technology, such clusters are often inaccessible.
“Bacteria are complicated, and just because we can sequence a gene doesn’t mean we know how the bacteria would turn it on to produce proteins,” Brady says. “There are thousands and thousands of uncharacterized gene clusters, and we have only ever figured out how to activate a fraction of them.” Frustrated with their inability to unlock many bacterial gene clusters, Brady and colleagues turned to algorithms. By teasing apart the genetic instructions within a DNA sequence, modern algorithms can predict the structure of the antibiotic-like compounds that a bacterium with these sequences would produce. Organic chemists can then take that data and synthesize the predicted structure in the lab.
It may not always be a perfect prediction. “The molecule that we end up with is presumably, but not necessarily, what those genes would produce in nature,” Brady says. “We aren’t concerned if it is not exactly right we only need the synthetic molecule to be close enough that it acts similarly to the compound that evolved in nature.” Postdoctoral associates Zonggiang Wang and Bimal Koirala from the Brady lab began by searching through an enormous genetic-sequence database for promising bacterial genes that were predicted to be involved in killing other bacteria and hadn’t been examined previously. The “cil” gene cluster, which had not yet been explored in this context, stood out for its proximity to other genes involved in making antibiotics. The researchers duly fed its relevant sequences into an algorithm, which proposed a handful of compounds that cil likely produces. One compound, aptly dubbed cilagicin, turned out to be an active antibiotic.
Source: This news is originally published by scitechdaily